Journal: American Journal of Translational Research

Article Title: Hydroxysafflor yellow A mitigates myocardial fibrosis induced by isoproterenol and angiotensin II

doi:

Figure Lengend Snippet: Effects of HSYA on the migration and collagen synthesis of CFs in the presence of Ang II. A, B. The migration of CFs at the 0th and 24th h in the wound-healing assay and the migration rate. Scale bar: 200 μm. C-E. Expressions of Collagen I and Collagen III determined by Western blotting (normalized to GAPDH). Data presented as mean ± SEM, n = 3 per group. *P < 0.05, **P < 0.01, ***P < 0.001. HSYA, Hydroxysafflor Yellow A; CFs, Cardiac Fibroblasts; Ang II, Angiotensin II; GAPDH, Lyceraldehyde-3-Phosphate Dehydrogenase; SEM, Standard Error of Mean; ns, not significant.

Article Snippet: PVDF membranes were probed with antibodies to collagen I (1:1000, bs-10423R, Bioss), collagen III (1:1000, bs-0549R, Bioss), TGF-β1 (1:500, BM3901, Boster), Smad2/3 (1:500, E-AB-32920, Elabscience, Wuhan, Hubei, China), P-Smad2/3 (1:500, E-AB-21040, Elabscience), IL-1β (1:1000, ab9722, Abcam), IL-18 (1:800, BA14935, Boster), NLRP3 (1:800, ab214185, Abcam), ASC (1:500, bs-6741R, Bioss), cleaved caspase-1 (1:100, sc-56036, Santa Cruz, CA, USA), and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 1:2000, TA-08, ZSGB-BIO, Beijing, China) at 4°C overnight.

Techniques: Migration, Wound Healing Assay, Western Blot

Journal: Frontiers in Immunology

Article Title: IL-22 regulates endometrial regeneration by enhancing tight junctions and orchestrating extracellular matrix

doi: 10.3389/fimmu.2022.955576

Figure Lengend Snippet: Antibodies.

Article Snippet: 8 , Collagen-3, rabbit polyclonal , Bioss, MA, USA , bs-0948R , 1:1,000.

Techniques: Concentration Assay